Impact of Different Nutrient Enrichment Concentrations on the Growth of Microalga Nannochloropsis sp. (Monodopsidaceae) Culture
DOI:
https://doi.org/10.29329/actanatsci.2023.353.09Keywords:
Cell density, Growth, Microalgae, Nannochloropsis sp., Nutrient enrichmentAbstract
Microalgae consist of unicellular algal species that can produce and accumulate a wide variety of biomolecules. In order to maintain a high cell density in a continuous phototrophic culture of algae, the nutrient can serve as the most important factor in enhancing cell density. In this study, the effect of different concentrations of nutrients on the cell density of microalga Nannochloropsis sp. cultured in the mega plastic box (with 50 L capacity) was investigated. Four groups of treatment with four replicates were tested: group A (including 5 g L-1 of ferric chloride, ammonium phosphate, and Urea), group B (including 10 g L-1 of ferric chloride, ammonium phosphate, and urea), group C (including 15 g L-1 of ferric chloride, ammonium phosphate, and urea), and group D (including 15 g L-1 of ferric chloride, ammonium phosphate, and urea with 40 g L-1 of cow manure). Results revealed that group C and group D achieved maximum density on day three as 86.39×106 cell mL-1 and 85.59×106 cell mL-1, respectively, which were significantly (p≤0.05) higher than the cell density of groups A (58.01×106 cell mL-1) and group B (70.67×106 cell mL-1). Additionally, the increasing specific growth rate (SGR) of Nannochloropsis sp. cultured was obtained in group D at 0.308 day-1 after the culture period. From the result of the study, it is concluded that the concentrations of 15 g L-1 ferric chloride, ammonium phosphate, and urea (group C) and 15 g L-1 ferric chloride, ammonium phosphate, and urea combined with 40 g L-1 cow manure (group D) are capable of increasing cell density growth of microalga Nannochloropsis sp. cultured in a mega plastic box.
References
Barkia, I., Saari, N., & Manning, S. R. (2019). Microalgae for high-value products towards human health and nutrition. Marine Drugs, 17(5), 304. https://doi.org/10.3390/md17050304
Benner, P., Meier, L., Pfeffer, A., Krüger, K., Oropeza Vargas, J. E., & Weuster-Botz, D. (2022). Lab-scale photobioreactor systems: Principles, applications, and scalability. Bioprocess and Biosystems Engineering, 45(5), 791-813. https://doi.org/10.1007/s00449-022-02711-1
Bogaard, A., Heaton, T. H., Poulton, P., & Merbach, I. (2007). The impact of manuring on nitrogen isotope ratios in cereals: archaeological implications for reconstruction of diet and crop management practices. Journal of Archaeological Science, 34(3), 335-343. https://doi.org/10.1016/j.jas.2006.04.009
Chidambara-Murthy, K. N., Vanitha, A., Rajesha, J., Mahadeva-Swamy, M., Sowmya, P. R., & Ravishankar, G. A. (2005). In vivo antioxidant activity of carotenoids from Dunaliella salina—a green microalga. Life Sciences, 76(12), 1381-1390. https://doi.org/10.1016/j.lfs.2004.10.015
Chu, W. L. (2012). Biotechnological applications of microalgae, International e-Journal of Science, Medicine & Education, 6(Suppl 1), S24-S37.
Ciccone, M. M., Cortese, F., Gesualdo, M., Carbonara, S., Zito, A., Ricci, G., De Pascalis, F., Scicchitano, P., & Riccioni, G. (2013). Dietary intake of carotenoids and their antioxidant and anti-inflammatory effects in cardiovascular care. Mediators of Inflammation, 2013, 782137. https://doi.org/10.1155/2013/782137
Dixit, R.B., & Suseela, M. R. (2013). Cyanobacteria: Potential candidates for drug discovery. Antonie Van Leeuwenhoek. 103(5), 947-961. https://doi.org/10.1007/s10482-013-9898-0
Duong, V. T., Li, Y., Nowak, E., & Schenk, P. M. (2012). Microalgae isolation and selection for prospective biodiesel production. Energies, 5(6), 1835-1849. https://doi.org/10.3390/en5061835
Durmaz, Y., & Erbil, G.Ç. (2017). Effect of light path length of tubes on growth rate of Nannochloropsis oculata using industrial scale tubular photobioreactor in the marine hatchery. Fresenius Environmental Bulletin, 26(7), 4783-4789.
Durmaz, Y. (2007). Vitamin E (α-tocopherol) production by the marine microalgae Nannochloropsis oculata (Eustigmatophyceae) in nitrogen limitation. Aquaculture, 272(1-4), 717-722. https://doi.org/10.1016/j.aquaculture.2007.07.213
Durmaz, Y., & Erbil, G. Ç. (2020). Comparison of industrial-scale tubular photobioreactor to FRP (Fiberglass reinforced plastic) panel photobioreactor on outdoor culture of Nannochloropsis oculata in the marine hatchery. Ege Journal of Fisheries and Aquatic Sciences, 37(4), 303-308. https://doi.org/10.12714/egejfas.37.3.13
Erbil, G. Ç., & Durmaz, Y. (2020). Effects of myo-inositol concentration on growth and pigments of Nannochloropsis oculata culture. Ege Journal of Fisheries and Aquatic Sciences, 37(2), 195-199. https://doi.org/10.12714/egejfas.37.2.11
Erbil, G. C., Elp, M., & Durmaz, Y. (2022). Effect of ferric chloride (FeCl3) concentration on pigment production of Porphyridium cruentum. International Aquatic Research, 14(2), 127-137. https://doi.org/10.22034/iar.2022.1950929.1234
Fenton, O. (2012). Agricultural nutrient surpluses as potential input sources to grow third generation biomass (microalgae): A review. Algal Research, 1(1), 49-56. https://doi.org/10.1016/j.algal.2012.03.003
Garcı́a-González, A., & Ochoa, J. L. (1999). Anti-inflammatory activity of Debaryomyces hansenii Cu, Zn-SOD. Archives of Medical Research, 30(1), 69-73. https://doi.org/10.1016/S0188-0128(98)00005-0
George, B., Pancha, I., Desai, C., Chokshi, K., Paliwal, C., Ghosh, T., & Mishra, S. (2014). Effects of different media composition, light intensity and photoperiod on morphology and physiology of freshwater microalgae Ankistrodesmus falcatus–A potential strain for bio-fuel production. Bioresource Technology, 171, 367-374. https://doi.org/10.1016/j.biortech.2014.08.086
Giwa, A. (2017). Comparative cradle-to-grave life cycle assessment of biogas production from marine algae and cattle manure biorefineries. Bioresource Technology, 244, 1470-1479. https://doi.org/10.1016/j.biortech.2017.05.143
Guzman, S., Gato, A., & Calleja, J. M. (2001). Anti-inflammatory, analgesic and free radical scavenging activities of the marine microalgae Chlorella stigmatophora and Phaeodactylum tricornutum. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, 15(3), 224-230. https://doi.org/10.1002/ptr.715
Hejazi, M.A., Holwerda, E., & Wijffels, R.H. (2004). Milking microalga Dunaliella salina for β‐carotene production in two‐phase bioreactors. Biotechnology and Bioengineering, 85(5), 475-481. https://doi.org/10.1002/bit.10914
Huang, X., Huang, Z., Wen, W., & Yan, J. (2013). Effects of nitrogen supplementation of the culture medium on the growth, total lipid content and fatty acid profiles of three microalgae (Tetraselmis subcordiformis, Nannochloropsis oculata and Pavlova viridis). Journal of Applied Phycology, 25, 129-137. https://doi.org/10.1007/s10811-012-9846-9
Huleihel, M., Ishanu, V., Tal, J., & Arad, S. M. (2001). Antiviral effect of red microalgal polysaccharides on Herpes simplex and Varicella zoster viruses. Journal of Applied Phycology, 13(2), 127-134. https://doi.org/10.1023/A:1011178225912
Jjemba, P. K. (2002). The potential impact of veterinary and human therapeutic agents in manure and biosolids on plants grown on arable land: a review. Agriculture, Ecosystems & Environment, 93(1-3), 267-278.
Juneja, A., Ceballos, R. M., & Murthy, G. S. (2013). Effects of environmental factors and nutrient availability on the biochemical composition of algae for biofuels production: A review. Energies, 6(9), 4607-4638. https://doi.org/10.3390/en6094607
Lebeau, T., & Robert, J. M. (2003). Diatom cultivation and biotechnologically relevant products. Part I: Cultivation at various scales. Applied Microbiology and Biotechnology, 60, 612-623. https://doi.org/10.1007/s00253-002-1176-4
Leu, S., & Boussiba, S. (2014). Advances in the production of high-value products by microalgae. Industrial Biotechnology, 10(3), 169-183. https://doi.org/10.1089/ind.2013.0039
Low, C., & Toledo, M. I. (2015). Assessment of the shelf-life of Nannochloropsis oculata flocculates stored at different temperatures. Latin American Journal of Aquatic Research, 43(2), 315-321. https://doi.org/10.3856/vol43-issue2-fulltext-7
Lu, Q., & Xiao, Y. (2022). From manure to high-value fertilizer: The employment of microalgae as a nutrient carrier for sustainable agriculture. Algal Research, 67, 102855. https://doi.org/10.1016/j.algal.2022.102855
Mohan, S. V., Rohit, M. V., Chiranjeevi, P., Chandra, R., & Navaneeth, B. (2015). Heterotrophic microalgae cultivation to synergize biodiesel production with waste remediation: progress and perspectives. Bioresource Technology, 184, 169-178. https://doi.org/10.1016/j.biortech.2014.10.056
Mortensen, A. (2006). Carotenoids and other pigments as natural colorants. Pure and Applied Chemistry, 78(8), 1477-1491. https://doi.org/10.1351/pac200678081477
Pulz, O., & Gross, W. (2004). Valuable products from biotechnology of microalgae. Applied Microbiology and Biotechnology, 65(6), 635-648. https://doi.org/10.1007/s00253-004-1647-x
Skulberg, O. M. (2000). Microalgae as a source of bioactive molecules–experience from cyanophyte research. Journal of Applied Phycology, 12(3), 341-348. https://doi.org/10.1023/A:1008140403621
Souza, C. M. M., Bastos, T. S., & dos Santos, M. C. (2021). Microalgae use in animal nutrition. Research, Society and Development, 10(16), e53101622986. https://doi.org/10.33448/rsd-v10i16.22986
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Copyright (c) 2023 Norsheen B. Sanuddin, Melodina D. Hairol, Cherry T. Nian, Rizal Jhunn F. Robles, Hadjiran A. Illud, Jubail S. Muyong, Jamrun H. Ebbah, Jurmin H. Sarri
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