Effect of Using Tobacco and Cloves Anesthesia in Fish Harvest on the Stress Parameters and Shelf Life of Rainbow Trout Fillet
DOI:
https://doi.org/10.61326/actanatsci.v6i1.342Keywords:
Rainbow trout, Clove, Tobacco, Stress parameters, Fillet shelf lifeAbstract
In this survey, we compare the effect of two anesthetics, clove and tobacco, on the harvest stress and quality of rainbow trout fillet kept at 4°C for 7 days. The fish sample groups (weighed 200 g) included a control, a group anesthetized with clove (750 mg/l) and a group anesthetized with tobacco (800 mg/l). The results showed that anesthesia with tobacco and clove significantly affects blood lactate and glucose (p<0.05). Fish anesthetized with clove showed less stress during harvesting. Also, the amount of glucose and lactate in their blood was less than those anesthetized with tobacco. There were also some significant changes in Thiobarbituric acid (TBA) and Total Volatile Basic Nitrogen (TVB-N) parameters during the storage of samples in the fridge. The lowest amount of Thiobarbituric acid, Total Volatile Basic Nitrogen, mesophilic, and psychrophilic bacteria during the survey belonged to samples treated with clove. The results also suggest that the fish anesthetized with clove stayed fresh longer in the fridge.
References
Agokei, O. E., & Adebisi, A. A. (2010). Tobacco as an anesthetic for fish handling procedures. Journal of Medicinal Plants Research, 4(14), 1396-1399.
AOAC. (2002). Official methods of analysis of the Association of the official Analysis Chemists. Association of Official Analytical Chemists (14th ed.), Washington, DC.
Arannilewa, S. T., Salawu, S. O., Sorungbe, A. A., & Ola-Salawu, B. B. (2005). Effect of frozen period on the chemical, microbiological and sensory quality of frozen tilapia fish (Sarotherodun galiaenus). African Journal of Biotechnology, 4(8), 852-855. https://doi.org/10.5897/AJB2005.000-3171
Aubourg, S. P. (2000). Assessment of antioxidant effectiveness on thermally treated marine Lipids by fluorescence detection. European Food Research and Technology, 211, 310-315. https://doi.org/10.1007/s002170000163
Chytiri, S., Chouliara, I., Savvaidis, I. N., & Kontominas, M. G. (2004). Microbiological, chemical and sensory assessment of iced whole and filleted aquaculture rainbow trout. Food Microbiology, 21(2), 157–165. https://doi.org/10.1016/S0740-0020(03)00059-5
de Azevado Gomes, H. A., da Silva, E. N., do Nascimanto, M. R. L., & Fukuma, H. T. (2003). Evaluation of the 2- thiobarbituric acid method for the measurement of lipid oxidation in mechanically deboned gamma irradiated chicken meat. Food Chemistry, 80(3), 433-437. https://doi.org/10.1016/S0308-8146(02)00499-5
Duran, A., Erdemli, U., Karakya, M., & Yilmaz, T. M. (2008). Effects of slaughter methods on physical, biochemical and microbiological quality of rainbow trout Oncorhynchus mykiss and mirror carp Cyprinus carpio filleted in pre-, in- or post-rigor periods. Fisheries Science, 74(5), 1146-1156. https://doi.org/10.1111/j.1444-2906.2008.01634.x
Erikson, U., & Misimi, E. (2008). Atlantic salmon skin and fillet color changes effected by perimortem handling stress, rigor mortis, and ice storage. Journal of Food Science, 73(2), 50-59. https://doi.org/10.1111/j.1750-3841.2007.00617.x
Gokoglu, N., Yerlikaya, P., & Cengiz, E. (2004). Effects of cooking methods on the proximate composition and mineral contents of rainbow trout (Oncorhynchus mykiss). Food Chemistry, 84(1), 19-22. https://doi.org/10.1016/S0308-8146(03)00161-4
Golomazou, E., Malandrakis, E. E., Kavouras, M., Karatzinos, T., Miliou, H., Exadactylos, A., & Panagiotaki, P. (2016). Anaesthetic and genotoxic effect of medicinal plant extracts in gilthead seabream (Sparus aurata L.). Aquaculture, 464, 673-682. https://doi.org/10.1016/j.aquaculture.2016.08.017
Gram, L., & Huss, H. H. (1996). Microbiological spoilage of fish and fish products. International Journal of Food Microbiology, 33(1), 121-137. https://doi.org/10.1016/0168-1605(96)01134-8
Hoseini, S. M., Taheri Mirghaed, A., & Yousefi, M. (2019). Application of herbal anaesthetics in aquaculture. Reviews in Aquaculture, 11(3), 550-564. https://doi.org/10.1111/raq.12245
Khosravizadeh M., & Romiani, L. (2019). Comparing chemical composition, textural and color properties of (Acanthopagrus morrisoni) under modified atmosphere packaging at -18°C, Journal of Marine Science and Technology, 17(4), 10-21. https://doi.org/10.22113/jmst.2017.50241
Lacerda, L. A., Oliveira, S. T., Pedralli, V., & González, F. H. D. (2009). Glucose and Lactate in Canine Serum and NAF/EDTA-NA2 Plasma samples: Implications of storage time. World Small Animal Veterinary Association World Congress Proceedings, São Paulo, Brazil.
Lago, H., Pena, J., & Aubourg, S. P. (2010). Effect of Slaughtering conditions on Lipid damage of chilled farmed turbot (Psetta maxima) muscle. Grasas Y Aceites, 61(3), 312-320. https://doi.org/10.3989/gya.123009
Maeda, T., Yaguchi, S., Fukushima, H., Harada, K., & Fukuda, Y. (2014). Post-catch fish handling for high quality fish products. Journal of National Fisheries University, 62(4), 155-158.
Marking, L. L., & Meyer, F. P. (1985). Are better anesthetics needed in fisheries? Fisheries, 10(6), 2-5. https://doi.org/10.1577/1548-8446(1985)010%3C0002:ABANIF%3E2.0.CO;2
Moini, S., Mouloodi, Z., & Shabanpour, B. (2011). Effects of stunning by ice and clove oil, and asphyxia on stress responses and quality indicators in common carp (Cyprinus carpio). Journal of Fisheries, 64(2-2), 153-162.
Ojagh, S. M., Rezaei, M., Razavi, S. H., & Hosseini, S. V. (2010). Effect of chitosan coatings enriched with cinnamon oil on the quality of refrigerated rainbow trout. Food Chemistry, 120(1), 193-198. https://doi.org/10.1016/j.foodchem.2009.10.006
Özyurt, G., Kuley, E., Özkütük, S., & Özogul, F. (2009). Sensory, microbiological and chemical assessment of the freshness of red mullet (Mullus barbatus) and goldband goatfish (Upeneus moluccensis) during storage in ice. Food Chemistry, 114(2), 505-510. https://doi.org/10.1016/j.foodchem.2008.09.078
Perez-Alonso, F., Aubourg, S.P., Rodriguez, O., & Barros- Velazquez, J. (2004). Shelf life extension of Atlantic pomfret (Brama brama) fillets by packaging under a vacuum- skin system. Journal of food Research Technological, 218, 313-317. https://doi.org/10.1007/s00217-003-0831-z
Rahmanifarah, K., Moloodi, Z., Moini, S., Shabanpour, B., Shabani, A., & Imanpour, M. R. (2010). Effects of CO2, clove oil stunning procedure and asphyxia on meat quality in common carp (Cyprinus carpio). Journal of Veterinary Research, 65(4), 301-306.
Salari-Joo, H., Kalbassi, M. R., & Abdollahzadeh, E. (2012). Effect of silver nanoparticles on bacterial flora of rainbow trout (Oncorhynchus mykiss) skin. Journal of Oceanography, 3(11), 83-90.
Sink, T. D., Strange, R. J., & Sawyers, R. E. (2007). Clove oil used at lower concentrations is less effective than ms-222 at reducing cortisol stress responses in anesthetized rainbow trout. North American journal of Fisheries Management, 27(1), 156-167. https://doi.org/10.1577/M05-103.1
Stoskopf, M., & Posner, L. M. (2008). Anesthesia and restraint of laboratory fish. In R. E. Fish, M. J. Brown, P. J. Danneman & A. Z. Karas (Eds.), Anesthesia and analgesia in laboratory animals (pp. 519-533). Academic press. https://doi.org/10.1016/B978-012373898-1.50025-5
Summerfelt, R. C., & Smith, L.S. (1990). Anaesthetic surgery and related Techniques. In C. B. Shreck & P. B. Moyle (Eds.), Methods for biology (pp. 213- 237). American Fisheries Society.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Sirvan Broumand, Ali Parsakhaghah, Erkan Can, Şafak Seyhaneyıldız Can

This work is licensed under a Creative Commons Attribution 4.0 International License.
