Effect of Spraying Sulfur and Inoculation Rhizobacteria on Growth and Yield of Canola
DOI:
https://doi.org/10.61326/actanatsci.v6i2.365Keywords:
Canola, Microorganisms, SulfurAbstract
Due to the increasing importance of using environmentally friendly methods to increase the yield of crops, two- year experiment carried out in a field located in the Dehgolan region, in the northwest of Iran, to study influence of sulfur spraying and plant growth promoting rhizobacteria inoculation on canola traits. The experiment was arranged as a split-plot factorial arrangement based on randomized complete block design with three replications. The main plots included two levels of sulfur (control and application), and the factorial combinations of strigolactone (control and application) and microorganisms (control, Funneliformis mosseae, Bacillus lentus, Pseudomonas fluorescens, Thiobacillus sp.) were allocated to the sub-plots. The results of combined analysis showed that the 1000 seed weights and seed yield increased significantly by sulfur application. At the same time, the 1000 seed weights decreased under the influence of strigolactone. Also, the SPAD number, the number of pods and seeds per plant, the 1000 seed weights and the seed yield increased significantly by influence of microorganisms, especially Thiobacillus, compared to control treatment. Based on the results of interaction effects, all traits except the number of SPAD were affected by the interaction effect of sulfur, strigolactone and microorganisms. The application of sulfur along with strigolactone and Thiobacillus significantly increased the number of pods per plant (200), the 1000 seed weights (4.53 g) and the seed yield (2552 kg/ha).
References
Alvi, A. F., Sehar, Z., Fatma, M., Masood, A., & Khan, N. A. (2022). Strigolactone: An emerging growth regulator for developing resilience in plants. Plants, 11(19), 2604. https://doi.org/10.3390/plants11192604
Asadi Rahmani, H., Khavazi Jahandideh, K., Mahjen Abadi, V. A., Ramezanpour, M. R., Mirzapour, M. H., & Mirzashahi, K. (2018). Effect of thiobacillus, sulfur, and phosphorus on the yield and nutrient uptake of canola and the chemical properties of calcareous soils in Iran. Communications in Soil Science and Plant Analysis, 49(14), 1671-1683. https://doi.org/10.1080/00103624.2018.1474905
Baghaie, A. H. (2023). Effect of sulfur granular municipal solid waste, humic acid, and nano Fe-oxide on lead uptake by plants in a calcareous soil in the presence of Thiobacillus. Avicenna Journal of Environmental Health Engineering, 10(1), 10-17. https://doi.org/10.34172/ajehe.2023.5284
Begum, N., Qin, C., Ahanger, M. A., Raza, S., Khan, M. I., Ashraf, M., & Zhang, L. (2019). Role of arbuscular mycorrhizal fungi in plant growth regulation: implications in abiotic stress tolerance. Frontiers in Plant Science, 10, 1068. https://doi.org/10.3389/fpls.2019.01068
Bremner, J. M., & Mulvaney, C. S. (1982). Nitrogen-total. In A. L. Page, R. H. Miller & D. R. Keeney (Eds.), Methods of soil analysis. Part 2: Chemical and microbiological properties (pp. 595-624). American Society of Agronomy, Soil Science Society of America.
Burr, A. H. (1984). Evolution of eyes and photoreceptor organelles in the lower phyla. In M. A. Ali (Ed.), Photoreception and vision in invertebrates. NATO Science Series A: Life Sciences, volume 74 (pp. 131-178). Springer. https://doi.org/10.1007/978-1-4613-2743-1_5
Choi, N. H., Choi, G. J., Min, B. S., Jang, K. S., Choi, Y. H., Kang, M. S., Park, M. S., Choi, J. E., Bae, B. K., & Kim, J. C. (2009). Effects of neolignans from the stem bark of Magnolia obovata on plant pathogenic fungi. Journal of Applied Microbiology, 106(6), 2057-2063. https://doi.org/10.1111/j.1365-2672.2009.04175.x
Gryndler, M., Hršelová, H., Chvátalová, I., & Vosátka, M. (1998). In vitro profileration of Glomus fistulosum intraradical hyphae from mycorrhizal root segments of maize. Mycological Research, 102(9), 1067-1073. https://doi.org/10.1017/S0953756297006060
Hamadali, H., Hafidi, M., Virolle, M. J., & Ouhdouch, Y. (2008). Rock phosphate-solubilizing Actinomycetes: Screening for plant growth-promoting activities. World Journal of Microbiology and Biotechnology, 24(11), 2565-2575. https://doi.org/10.1007/s11274-008-9817-0
Karaaslan, D., Toncer, O., & Ozturk, F. (2020). The effect of different sulfur levels on seed yield and oil content of some rapeseed cultivars. Journal of Agronomy, Technology and Engineering Management, 3(2), 402-407.
Kazemi Oskuei, B., Bandehagh, A., Farajzadeh, D., Asgari Lajayer, B., Shu, W., & Astatkie, T. (2023). Effects of Pseudomonas fluorescens FY32 on canola (Brassica napus L.) cultivars under drought stress induced by polyethylene glycol. Journal of Crop Health, 76, 251-260. https://doi.org/10.1007/s10343-023-00958-6
López-Valdez, F., Fernández-Luqueño, F., Ceballos-Ramírez, J. M., Marsch, R., Olalde-Portugal, V., & Dendooven, L. (2011). A strain of Bacillus subtilis stimulates sunflower growth (Helianthus annuus L.) temporarily. Scientia Horticulturae, 128(4), 499-505. https://doi.org/10.1016/j.scienta.2011.02.006
Ma, B. L., Biswas, D. K., Herath, A. W., Whalen, J. K., Qianying Ruan, S., Caldwell, C., Earl, H., Vanasse, A., Scott, P., & Smith, D. L. (2015). Growth, yield, and yield components of canola as affected by nitrogen, sulfur, and boron application. Journal of Plant Nutrition and Soil Science, 178(4), 658-670. https://doi.org/10.1002/jpln.201400280
Ma, N., Hu, C., Wan, L., Hu, Q., Xiong, J., Zhang, C. (2017). Strigolactones improves plant growth, photosynthesis, and alleviates oxidative stress under salinity in rapeseed (Brassica napus L.) by regulating gene expression. Frontiers in Plant Science, 8, 1671. https://doi.org/10.3389/fpls.2017.01671
Mitra, D., Uniyal, N., Panneerselvam, P., Senapati, A., & Ganeshamurthy, A. (2019). Role of mycorrhiza and its associated bacteria on plant growth promotion and nutrient management in sustainable agriculture. International Journal of Life Sciences and Applied Sciences, 1, 1-10.
Mohamed, H. I., & Gomaa, E. Z. (2012). Effect of plant growth promoting Bacillus subtilis and Pseudomonas fluorescens on growth and pigment composition of radish plants (Raphanus sativus) under NaCl stress. Photosynthetica, 50(2), 263-272. https://doi.org/10.1007/s11099-012-0032-8
Mohammadi, K., Heidari, G. R., Karimi Nezhad, M. T., Ghamari, S., & Sohrabi, Y. (2012). Contrasting soil microbial responses to fertilization and tillage systems in canola rhizosphere. Saudi Journal of Biological Sciences, 19(3), 377-383. https://doi.org/10.1016/j.sjbs.2012.05.001
Motamed, M., Firoozabadi, M., & Sinaki, J. (2018). Effects of Thiobacillus, sulfur and micronutrient spray on some traits of green beans. Journal of Chemical Health Risks, 8(3), 209-216.
Mukherjee, A., & Ane, J. M. (2011). Germinating spore exudates from arbuscular mycorrhizal fungi: molecular and developmental responses in plants and their regulation by ethylene. Molecular Plant-Microbe Interactions, 24(2), 260-270. https://doi.org/10.1094/mpmi-06-10-0146
Nacoon, S., Jogloy, S., Riddech, N., & Mongkolthanaruk, W. (2020). Interaction between phosphate solubilizing bacteria and arbuscular mycorrhizal fungi on growth promotion and tuber inulin content of Helianthus tuberosus L. Scientific Reports, 10, 4916. https://doi.org/10.1038/s41598-020-61846-x
Neshat, M. R., Dadashi Chavan, D., Shirmohammadi, E., Pourbabaee, A. A., Zamani, F., & Torkaman, Z. (2023). Canola inoculation with Pseudomonas baetica R27N3 under salt stress condition improved antioxidant defense and increased expression of salt resistance elements. Industrial Crops and Products, 206(15), 117648. https://doi.org/10.1016/j.indcrop.2023.117648
Olsen, S.R., & Sommers, L.E. (1982). Phosphorus. In A. L. Page (Ed.), Methods of soil analysis: Part 2 Chemical and microbiological properties, 9.2.2, Second Edition (pp. 403-430). American Society of Agronomy, Soil Science Society of America.
Poisson, E., Trouverie, J., Brunel-Muguet, S., Akmouche, Y., Pontet, C., Pinochet, X., & Avice, J. C. (2019). Seed yield components and seed quality of oilseed rape are impacted by sulfur fertilization and its interactions with nitrogen fertilization. Frontiers in Plant Science, 10, 458. https://doi.org/10.3389/fpls.2019.00458
Pużyńska, K., Kulig, B., Halecki, W., Lepiarczyk, A., & Pużyński, S. (2018). Response of oilseed rape leaves to sulfur and boron foliar application. Acta Physiologiae Plantarum, 40, 169. https://doi.org/10.1007/s11738-018-2748-y
Rameeh, V., Niakan, M., & Mohammadi, M. H. (2019). Sulfur effects on sugar content, enzyme activity and seed yield of rapeseed (Brassica napus L.). Agronomia Colombiana, 37(3), 311-316. https://doi.org/10.15446/agron.colomb.v37n3.71830
Schreiner, R. P, & Koide, R. T. (1993). Antifungal compounds from the roots of mycotrophic and non-mycotrophic plant species. New Phytologist, 123(1), 99-105. https://doi.org/10.1111/j.1469-8137.1993.tb04535.x
Shoja, T., Majidian, M., & Rabiee, M. (2018). Effects of zinc, boron and sulfur on grain yield, activity of some antioxidant enzymes and fatty acid composition of rapeseed (Brassica napus L.). Acta Agriculturae Slovenica, 111(1), 73-84. https://doi.org/10.14720/aas.2018.111.1.08
Singh, A., Singh, A., Rexer, K. H., Kost, G., & Varma, A. (2003). Root endosymbiont: Piriformospora indica – a boon for orchids. The Journal of Orchid Society of India, 15, 89-102.
Souchie, E. L., Azcón, R., Barea, J. M., Saggin-Júnior, O. J., & da Silva, E. M. R. (2007). Indolacetic acid production by P-solubilizing microorganisms and interaction with arbuscular mycorrhizal fungi. Acta Scientiarum. Biological Sciences, 29(3), 315-320.
Tabasi, A., Dadashi, M. R., & Faraji, A. (2017). Effect of different sulfur levels plus Thiobacillus on yield and yield components of canola (Brassica napus L.) cultivars in Gorgan, Iran. Azarian Journal of Agriculture, 4(3), 87-94.
Taurian, T., Anzuay, M. S., Angelini, J. G., Tonelli, M. L., Ludueña, L., Pena, D., Ibáñez, F., & Fabra, A. (2010). Phosphate-solubilizing peanut associated bacteria: Screening for plant growth-promoting activities. Plant and Soil, 329(1), 421-431. https://doi.org/10.1007/s11104-009-0168-x
Ur Rehman, H., Iqbal, Q., & Farooq, M. (2013). Sulphur application improves the growth, seed yield and oil quality of canola. Acta Physiologiae Plantarum, 35, 2999-3006. https://doi.org/10.1007/s11738-013-1331-9
Vishniac, W., & Santer, M. (1975). The thiobacilli. Bacteriological Reviews, 21(3), 195-213. https://doi.org/10.1128/br.21.3.195-213.1957
Wang, Y., Fang, B., Zhang, D., Yue, J., Yang, C., Simeng, D., Yunhui, S., Wang, H., Jin, H., & Li, X. (2022). Effects of exogenous Strigolactone on wheat grain filling and yield formation in different cultivation densities. Journal of Biobased Materials and Bioenergy, 16(4), 641-652. https://doi.org/10.1166/jbmb.2022.2205
Warncke, D., & Brown, J. R. (2011). Potassium and Other Basic Cations. In J. R. Brown (Ed.), Recommended chemical soil test procedures for the north central region (pp. 15–19). North Central Regional Research Publication No. 221.
Yang, L., Xie, J., Jiang, D., Fu, Y., Li, G., & Lin, F. (2008). Antifungal substances produced by Penicillium oxalicum strain PY-1—potential antibiotics against plant pathogenic fungi. World Journal of Microbiology and Biotechnology, 24(7), 909-915. https://doi.org/10.1007/s11274-007-9626-x
Yari, P., Pasari, B., Rokhzadi, A., & Mohammadi, K. (2022). Foliar application of silicon, sulfur, and flowering fruit set biostimulant on canola. Gesunde Pflanzen, 74, 193-203. https://doi.org/10.1007/s10343-021-00602-1
Yoneyama, K. (2020). Recent progress in the chemistry and biochemistry of strigolactones. Journal of Pesticide Science, 45(2), 45-53. https://doi.org/10.1584/jpestics.D19-084
Yoneyama, K., & Brewer, P. B. (2021). Strigolactones, how are they synthesized to regulate plant growth and development? Current Opinion in Plant Biology, 63, 102072. https://doi.org/10.1016/j.pbi.2021.102072
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