Growth and Chlorophyll Responses of Wheat Seedlings to Putrescine Under PEG-Induced Drought Stress

Authors

DOI:

https://doi.org/10.61326/actanatsci.v7i1.479

Keywords:

Gacer, Chlorophyll, PEG-6000, Putrescine, Triticum aestivum

Abstract

Wheat is one of the most important cereal crops worldwide due to its broad cultivation area, diverse uses, and economic importance. This study aimed to determine the shoot length, shoot fresh and dry weight, root length, root fresh and dry weight, chlorophyll a (chl a), chlorophyll b (chl b), total chlorophyll (chl a+b) amounts of wheat seedlings. The interactions of 5% polyethylene glycol (PEG-6000) and different putrescine (PUT) doses (0.5 mM and 1 mM) were examined in wheat (Triticum turgidum L. var. dicoccum, Gacer, Triticum aestivum L., Konya-2002) seedlings. Under the drought stress effect caused by PEG, two wheat varieties were negatively affected and a decrease in shoot length, fresh weight, dry weight and chlorophyll amounts was observed. In Gacer wheat, an increase in fresh root weight was observed under drought stress treated with PEG, while a decrease was observed in Konya-2002. Compared to the control, it was determined that the root length of Gacer wheat was less affected by the presence of PEG than that of Konya-2002. Compared to PEG, under the effect of PUT, increases were observed in shoot length, fresh weight, root length and fresh weight, chlorophyll a and total chlorophyll content in both wheat varieties. The applied 5% PEG-6000 created a drought stress effect in wheat and it was observed that PUT reduced this stress effect. When the parameters we used in our study are examined together, these findings suggest that Gacer wheat is more resilience to drought than Konya-2002.

References

Abdelmoghny, A. M., Raghavendra, K. P., & Sheeba, J. A. (2020). Morphophysiological and molecular characterization of drought tolerance traits in Gossypium hirsutum genotypes under drought stress. Physiology and Molecular Biology of Plants, 26, 2339–2353. https://doi.org/10.1007/s12298-020-00890-3

Alcázar, R., Marco, F., & Cuevas, J. C. (2006). Involvement of polyamines in plant response to abiotic stress. Biotechnology Letters, 28, 1867–1876. https://doi.org/10.1007/s10529-006-9179-3

Alharby, H. F., Al-Zahrani, H. S., Alzahrani, Y. M., Alsamadany, H., Hakeem, K. R., & Rady, M. M. (2021). Maize grain extract enriched with polyamines alleviates drought stress in Triticum aestivum through up-regulation of the ascorbate–glutathione cycle, glyoxalase system, and polyamine gene expression. Agronomy, 11(5), 949. https://doi.org/10.3390/agronomy11050949

Alobaidy, M. G. (2013). Effect of putrescine and humic acid on cotton plant growing under salinity stress conditions. American-Eurasian Journal of Agricultural & Environmental Sciences, 13(8), 1100–1110.

Arnon, D. I., & Hoagland, D. R. (1940). Crop production in artificial culture solutions and in soils with special reference to factors influencing yields and absorption of inorganic nutrients. Soil Science, 50(6), 463-485.

Atak, M. (2017). Buğday ve Türkiye buğday köy çeşitleri. Mustafa Kemal Üniversitesi Ziraat Fakültesi Dergisi, 22(2), 71–88.

Balkan, A., & Gençtan, T. (2023). Investigation of the effect of drought stress on some morphological and physiological traits in bread wheat (Triticum aestivum L.). MAS Journal of Applied Sciences, 9(1), 167–177. https://doi.org/10.5281/zenodo.10846009

Bukhari, M. A., Shah, A. N., Fahad, S., & Ikbal, J. (2021). Screening of wheat (Triticum aestivum L.) genotypes for drought tolerance using polyethylene glycol. Arabian Journal of Geosciences, 14, 2808. https://doi.org/10.1007/s12517-021-09073-0

Bulut, S. (2016). Hulled wheat farming in Develi. Current Trends in Natural Sciences, 5(9), 115–119.

Çömlekçioğlu, N., & Arıkan, S. (2017). Effects of physiological stress and exogenous polyamines on seedling growth and indigo amounts in Isatis tinctoria L. leaves. Mediterranean Agricultural Sciences, 30(3), 261–267. https://doi.org/10.29136/mediterranean.360003

Doğru, H., & Ergün, N. (2021). Effects of cadmium–salt interactions on growth and some genes in wheat. Applied Ecology and Environmental Research, 19(2), 1019–1031. https://doi.org/10.15666/aeer/1902_10191031

Hebat-Allah, A. H., Shifaa, A., Marwa, E. A., & Sahar, K. M. (2023). The promotive effect of putrescine on growth, biochemical constituents and yield of wheat (Triticum aestivum L.) plants under water stress. Agriculture, 13(3), 587. https://doi.org/10.3390/agriculture13030587

Hellal, F. A., El-Shabrawi, H. M., & El-Hady, M. (2018). Influence of PEG-induced drought stress on molecular and biochemical constituents and seedling growth of Egyptian barley cultivars. Journal of Genetic Engineering and Biotechnology, 16(1), 203–212. https://doi.org/10.1016/j.jgeb.2017.10.009

Hussain, S. S., Ali, M., Ahmad, M., & Siddique, K. H. M. (2011). Polyamines: Natural and engineered abiotic and biotic stress tolerance in plants. Biotechnology Advances, 29, 300–311. https://doi.org/10.1016/j.biotechadv.2011.01.003

İlhan, D., Koç, Ü. G., ... (2026). Effects of drought stress on Kars Kıvılca wheat (Triticum aestivum Schrank) populations. Journal of Tekirdag Agricultural Faculty, 23(3), 730–755. https://doi.org/10.33462/jotaf.1456547

İlyas, M., Nisar, M., & Han, N. (2021). Drought tolerance strategies in plants: A mechanistic approach. Journal of Plant Growth Regulation, 40(6), 926–944. https://doi.org/10.1007/s00344-020-10174-0

Kadıoğlu, A. (2006). Bitki fizyolojisi. Gündüz Ofset Matbaacılık ve Yayıncılık.

Kusano, T., Berberich, T., Tateda, C., & Takahashi, Y. (2008). Polyamines: Essential factors for growth and survival. Planta, 228, 367–381. https://doi.org/10.1007/s00425-008-0772-7

Money, N. P. (1989). Osmotic pressure of aqueous polyethylene glycols. Plant Physiology, 91, 766–769.

Özberk, İ., Atasay, S., & Altay, F. (2016). Türkiye'nin buğday atlası.

Pandey, A., Khan, M. K., & Thomas, G. (2015). Estimation of Indian and Turkish hexaploid wheat population structure employing molecular markers. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 43(1), 70–78. https://doi.org/10.15835/nbha4319835

Pekol, S., Baloğlu, M. C., & Altunoğlu, Y. Ç. (2016). Evaluation of genotoxic and cytologic effects of environmental stress in wheat species with different ploidy levels. Turkish Journal of Biology, 40(3), 580–588.

Porra, R. J., Thompson, W. A., & Kriedemann, P. E. (1989). Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents. Biochimica et Biophysica Acta, 975, 384–394. https://doi.org/10.1016/S0005-2728(89)80347-0

Rahman, A., Kulik, E., Majláth, I., Khan, I., Janda, T., & Pál, M. (2024). Different reactions of wheat, maize, and rice plants to putrescine treatment. Physiology and Molecular Biology of Plants, 30(5), 807–822. https://doi.org/10.1007/s12298-024-01462-5

Shi, H., Ye, T., & Chan, Z. (2013). Comparative proteomic and physiological analyses reveal the protective effect of exogenous polyamines in Bermuda grass (Cynodon dactylon) response to salt and drought stresses. Journal of Proteome Research, 12(11), 4951–4964. https://doi.org/10.1021/pr400479k

Shu, S., Guo, S. R., & Sun, J. (2012). Effects of salt stress on the structure and function of the photosynthetic apparatus in Cucumis sativus and its protection by exogenous putrescine. Physiologia Plantarum, 146, 285–296. https://doi.org/10.1111/j.1399-3054.2012.01623.x

Toraman, Ş. P., Ergün, N., & Çalıcı, B. (2020). Some abiotic stress on growth and lipid peroxidation on wheat seedlings. Natural and Engineering Sciences, 5(3), 144–154. https://doi.org/10.28978/nesciences.832975

Zhong, D., Yan, H., Chen, X., Zhong, Z., Li, X., Jia, X., Chang, S., Shen, J., & Zhang, D. (2025). Exogenous putrescine modulates variety-specific cadmium tolerance in wheat seedlings: Synergistic roles of antioxidant defense and physiological homeostasis. Frontiers in Plant Science, 16, 1600603. https://doi.org/10.3389/fpls.2025.1600603

Downloads

Published

2026-06-27

How to Cite

Şengül Toraman, P., Ergün, N., & Çalıcı, B. (2026). Growth and Chlorophyll Responses of Wheat Seedlings to Putrescine Under PEG-Induced Drought Stress. Acta Natura Et Scientia, 7(1), 85–95. https://doi.org/10.61326/actanatsci.v7i1.479

Issue

Section

Original Research Papers